The 10X P-Green Buffer allows you to go directly from thermal
cycler to gel analysis. The buffer contains a compound that
increases sample density, so that samples sink easily into the
wells of an agarose gel. The P-Green Buffer contains two dyes
(Yellow and Blue) that separate to allow easy monitoring during
electrophoresis. The blue dye migrates at the same rate as 3-5Kb
DNA fragments in a 1% agarose gel. The yellow dye migrates
ahead of primers (<50bp). Use the P-Green Buffer for direct-to-gel
analysis after amplification by fluorescence or absorbance without
prior purification of the DNA.
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